program

25th Meeting of the European Red Cell Society

From April 19-23, 2024 we have organized the 25^th meeting of the European Red Cell Society. The meeting was held at Hotel Noordsee, Ameland, the Netherlands.

This biannual International meeting attracts leading experts in the fields of erythropoiesis, red blood cell physiology and function, and red blood cell related disorders. It is an event that brings together 100+ people from Europe and beyond, and connects the fields of research, diagnostics and clinical implications. The target audience are researchers (basic and translational), laboratory specialists, hematologists, and PhD students.

The content of this 4 day course is the responsibility of the program committee, consisting of:
Dr. Emile van den Akker, dr. Robin van Bruggen, and dr. Marieke von Lindern (Sanquin, Amsterdam, The Netherlands)
Dr. Kees Harteveld (Leiden University Medical Center, Leiden, The Netherlands)
Dr. Marije Bartels, dr. Minke Rab, dr. Richard van Wijk (University Medical Center Utrecht, Utrecht, The Netherlands)

Friday, April 19

15:00 – 16:00

Outward journey Holwerd-Nes
16:45 – 17:30
- Check-in hotel
18:30 – 19:30

Cell cycle structure and speed in erythroid development and the response to erythroid stress
Merav Socolovsky, Professor UMASS Chan Medical School, United States
19:30 – 21:30

Buffet dinner

Saturday, April 20

07:30 – 08:45

Breakfast

Scientific session 1 – Erythropoiesies

Chairs: Wassim el Nemer / Catarina Freire

09:00 – 09:30

Erythromyeloblastic Islands
Lionel Blanc, Northwell, United States
09:30 – 09:45

Anemia links β-catenin with FOXO3 signaling during erythroid terminal differentiation
Santhe van der Meulen, Sanquin, the Netherlands
09:45 – 10:00

In vitro culture of erythroblasts (EBL) and production of mature erythrocytes for transfusions requires upscaling in fluidic-turbulent systems
Giulia Iacono, Sanquin, the Netherlands
10:00 – 10:15

The Role of the Zcchc6/Dis3L2 RNA Editor-Exonuclease Axis in Terminal Erythroid Differentiation
Areum Han, Harvard Medical school / Boston’s Children Hospital, United States
10:15 – 10:30

Transfusion-ready red blood cell generation from induced pluripoten stem cells (iPSCs)
Emile van den Akker, Sanquin, the Netherlands
10:30 – 10:45

Detailed characterization of iPSC-derived hematopoiesis and erythropoiesis en self-organized cellular complexes (hemanoids)
Isabel Dorn, Medical University Graz, Austria

10:45 – 11:15

Coffee break

Scientific session 2 – Ion channels of the red blood cell – function and disorders

Chairs: Giampaolo Minetti / Silvia Neri

11:15 – 11:45

Defects of RBC cell volume regulation and hydration state: their pathophysiology and diagnosis
Paola Bianchi, Fondazione IRCCS Ca’ Granda Policlinico Milan, Italy
11:45 – 12:00

Electrophysiological characterization of red blood cells using the automated patch clamp
Nicoletta Muarciano, Nanion Technologies, Germany
12:00 – 12:15

Role of Piezo 1 in terminal density reversal of healthy red blood cells
Kuntal Dey, University of Zurich, Switserland
12:15 – 12:30

Red Blood Cell Protein contribution to Malaria Parasite Invasion. Basigin mediation of Plasmodium falciparum red blood cell invasion does not require its transmembrane domain or interaction with monocarboxylate transporter
Timothy Satchwell, University Bristol, UK

12:30

Lunch
Networking session
Poster presentations – titles and authors can be found below this program
‘Meet the expert’ session with Ingolf Bernhardt
Unsolved problems in red cell research
Ingolf Bernhardt, University Saarland, Germany

Scientific session 3 – Red blood cell membrane and associated disorders & 4. Clinical hematology – new treatments in sickle cell disease and other hereditary red blood cell disorders

Chairs: Stephane Egee / Min Qiao

Red blood cell membrane and associated disorders

16:00 – 16:30

Membranopathies : from cytology and red cell/reticulocyte indices to molecular diagnosis, including prenatal diagnosis
Lydie da Costa, University Paris, France
16:30 – 16:45

Developmental changes in membrane lipid composition during terminal maturation of circulating human reticulocytes and ageing of red cells
Giampaolo Minetti, University of Pavia, Italy
16:45 – 17:00

Modulation of terminal erythropoiesis by a TSPO ligand
Alice Dussouchaud, Inserm, France
17:00 – 17:15

Complete absence of GLUT1 does not impair human terminal erythroid differentiation
Catarina Freire, University of Bristol, United Kingdom
17:15 – 17:30

Dual action of Dooku1 on PIEZO1 channel in human red blood cells
Guillaume Bouyer, CNRS, France

Clinical hematology – new treatments in sickle cell disease and other hereditary red blood cell disorders

17:30 – 17:45

Single center report of hemoglobinopathy free survival after pediatric hematopoietic stem cell transplantation
Gertjan Lugthart, pediatrician, LUMC, Leiden, the Netherlands

18:45 – 22:00

Dinner

Sunday, April 21

07:30 – 08:45
- Breakfast

Scientific session 4 – Clinical hematology – new treatments in sickle cell disease and other hereditary red blood cell disorders

Chairs: Sjaak Philipsen / Marissa Traets

09:00 – 09:30

Curative approaches for patients with sickle cell disease
Erfan Nur, Amsterdam UMC, the Netherlands
09:30 – 09:45

Genetic reversal of the globin switch concurrently modulates both fetal and sickle hemoglobin and reduces red cell sickling
Nicolas Hebert, Etablissement Francais Du Sand, France
09:45 – 10:00

Metabolic blood profile and response to treatment with the pyruvate kinase activator mitapivat in patients with sickle cell disease
Myrthe van Dijk, UMC Utrecht, the Netherlands
10:00 – 10:15

Plasma Proteome Profiling of Sickle Cell Patients Reveals Activation of Inflammatory Pathways During Steady State and Vaso-occlusive Crisis
Lydian de Ligt, Amsterdam UMC, the Netherlands
10:15 – 10:30

Red cell rheology and blood viscosity in individuals with sickle cell disease following allogenic bone marrow transplantation or autologous gene addition therapy
Vivien Sheehan, Emory University of Medicine, United States
10:30 – 10:45

A novel allosteric activator of pyruvate kinase enhances human and mouse enzymatic activity and leads to ematological improvements in sickle cell disease mouse models
Carsten Alt, Pfizer

10:45 – 11:15

Coffee break

Scientific session 5 – Disorders of Erythropoiesies

Chairs: Tim Satchwell / Carolina Hernandez

11:15 – 11:45

Patient-specific cell lines to study erythropoiesis and hemoglobin switching
Sjaak Philipsen, Erasmus MC Rotterdam, the Netherlands
11:45 – 12:00

Ineffective erythropoiesis negatively impacts the erythroblastic island in sickle cell disease
Wassim El Nemer, EFS Sante, France
12:00 – 12:15

Anemia in aged mice is accompanied by reduced erythroid lineage cell production and increased red blood cell life span
Carsten Alt, Pfizer, United States
12:15 – 12:30

Development of a beta-thalassemia rat model for bone marrow characterization after stem cell transplantation
Karolin Roemhild, Sanquin, the Netherlands
12:30 – 12:45

Elucidating glucocorticoid-responsiveness in Diamond-Blackfan anemia syndrome using iPSC-derived erythroblasts
Teun Slijkerman, Sanquin, the Netherlands

Scientific session 6 – New developments for red cell research and diagnostics

Chairs: Paola Bianchi / Jonathan de Wilde

09:00 – 09:30

Probing red blood cells’s bio-mechanical properties
Lars Kaestner, University of Saarland, Germany
09:30 – 09:45

An Efficient and Specific Reporter System Suitable for High-Throughput Screening of Novel Fetal Hemoglobin Inducers
Tingyue Li, ErasmusMC, Rotterdam, the Netherlands
09:45 – 10:00

Pyruvate Kinase Thermostability Is Associated with Red Blood Cell Adhesion, Deformability and Oxygen Affinity in Patients with Sickle Cell Disease
Marissa Traets, UMC Utrecht, the Netherlands
10:00 – 10:15

Towards quantification of intracellular free calcium in individual red blood cells by fluorescence lifetime imaging microscopy
Min Qiao, Saarland University, Germany
10:15 – 10:30

Ex vivo glycolytic flux measurements in red blood cells
Titine Ruiter, UMC Utrecht, the Netherlands
10:30 – 10:45

Hepatic Leukemia Factor as a marker for iPSC – derived Hematopoietic Stem Cells
Maria Simanovich, Erasmus MC Rotterdam, the Netherlands

10:45 – 11:15

Coffee break

Scientific session 7 – New developments for red cell research and diagnostics & 6. New developments for red cell research and diagnostics

Chairs: Lars Kaestner / Giulia Iacono

Clinical cases

11:15 – 11:45

SUPT5H nonsense variants as modifying factor in beta-thalassemia trait
Kees Harteveld, Leiden University MC, The Netherlands

New developments for red cell research and diagnostics

11:45 – 12:00

An AI-based imaging flow cytometry approach to study erythrophagocytosis
Silvia Neri, Sanquin, the Netherlands
12:00 – 12:15

Endothelialized 3D perfusion model to study ex vivo reticulocyte-to-erythrocyte transition
Claudia Maria Bernecker, University of Graz, Austria
12:15 – 12:30

Actomyosin-driven expulsion of hemoglobin during hemolysis,
Tessa Korsa, Fraunhofer IBMT, Sulzbach, Germany
12:30 – 12:45

Red cell function devices under development for use in sickle cell disease
Vivien Sheehan, Emory University School of Medicine, Atlanta, United States

12:45 – 13:45

Lunch

Scientific session 8 – Clinical hematology – new diagnostic approaches & 2. Ion Channels

Chairs: Vivian Sheehan / Myrthe van Dijk

13:45 – 14:15

Biomarkers in sickle cell disease
Minke Rab, UMC Utrecht, The Netherlands
14:15 – 14:30

Novel direct plasma heme-related assay decyphers hemolytic anemia and ineffective erythropoiesis
Nicolas Hebert, Etablissement Francais Du Sang IDF, France
14:30 – 14:45

Impact of Sickle Cell Disease Patients-Derived Red Blood Cells on Clot Dynamics and Fibrin Structure
Naoual Ouazzani Chahdi, Sanquin, the Netherlands
14:45 – 15:00

Low Voxelotor Concentrations Exacerbate Sickle Cell Formation At Low Oxygen Pressure
Emile van den Akker, Sanquin, the Netherlands
15:00 – 15:15

Soluble Transferrin Receptor as a Marker for Assessing Disease Severity in Hereditary Hemolytic Anemias
Geoffrey Kuppens, UMC Utrecht, the Netherlands
Presented by Marije Bartels, UMC Utrecht, the Netherlands
15:15 – 15:30

Autoimmune hemolytic anemia anti-RBC IgM, but not IgG, drive in vitro complement activation
Femke Mulder, Sanquin, the Netherlands

Ion Channels

15:30 – 15:45

Ion channels of the red blood cell:
Effects of Pyruvate Kinase Activation in Hereditary Hemolytic Anemia
Jonathan de Wilde, UMC Utrecht, the Netherlands

15:45 – 16:15

Coffee break, followed by sessions:
15:45 – 16:45

Poster presentations – titles and authors can be found under this program
15:45 – 17:00

Steering Committee meeting on the consensus-based Recommendation for the Diagnosis of Rare Hemolytic Anemia
Sponsored by EuroBloodNet
17:00 – 17:45

ERCS General Assembly meeting
18:30 – 21:30

Dinner

Tuesday, April 23

07:00 – 08:00

Breakfast and checkout, payment of expenses (bar)
08:30

Departure from hotel by bus to harbor
09:00

Departure by ferry ‘Brakzand’ to mainland

Posters, format A0, portrait

Pseudo Action Potentials (PAPs) in red blood cells – an approach of understanding
Lars Kaestner, University of Saarland, Germany
Anemia in aged mice is accompanied by reduced erythroid lineage cell production and increased red blood cell life span
Carsten Alt, Pfizer
A novel allosteric activator of pyruvate kinase enhances human and mouse enzymatic activity and leads to hematological improvements in sickle cell disease mouse models
Carsten Alt, Pfizer
Can the emergence of cation channel activity during storage threaten the survival of transfused red blood cells?
Stephane Egee, CNRS, France
Cell Therapy Scale-up: Bioprocess Development for the Production of Hematopoietic Cells
Brenda Juarez Garza, Delft University, the Netherlands
The quality of red blood cells isolated from cord blood during storage
Joyce Bestebroer, Sanquin, the Netherlands
Variability of extracellular particle content in red blood cell concentrates upon storage is associated with differential membrane alterations and could be used as quality control
Donatienne Tyteca, UC Louvain, Belgium
Hemoglobin as a multifunctional sensor and buffer
Anna Bogdanova, University of Zurich, Switzerland
Scale-up of stem cell cultures from shake flask to bioreactor: a CFD-based comparison of hydrodynamic stress
Ramon van Valderen, Delft University, the Netherlands
Towards an improved erythroid production from IPSC
Marien van der Stel, Sanquin, the Netherlands
Investigating the Role of Shear Stress on Endothelial-to-Hematopoietic Transitioning
Oyishee Ahmad, Sanquin, the Netherlands
A clinical mechanical marker of red blood cell deformability: sickle cell disease example
Cecile Jebane, CNRS-CINam, Marseille, France
RBC delivered antigens for the induction of antigen specific immune tolerance,
Youmna Al Halabi, Sanquin, the Netherlands

This conference is sponsored by